The hiseq 2500 system is a powerful highthroughput sequencing system. Illumina hiseq 1500 nextgen sequencer for sale online ebay. This new hiseq 2500 dataset, compares data quality and run time for a 2x100bp sequencing run on the hiseq 2000 and the hiseq 2500. Nextera rapid capture custom enrichment kit illumina. This user guide provides instructions for running hiseq analysis software v0. Library quantification kit user manual 050919 takara bio usa, inc. The first illumina nextgen sequencing platform, the genome analyzer developed by solexa, was launched in 2006. Comparative performance of the bgiseq500 vs illumina. Illumina hiseq 2000 sequencer 15007862 includes computer and software. The infinium asian screening array builds on the success of the infinium global screening array, which was developed by a community of researchers, healthcare networks, direct to consumer companies, and service providers.
Illumina hiseq 1500 genome dna sequencing system, cables, manuals, extras. Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational. A rehybridization run on the hiseq 2500 or hiseq 1500 repeats the sequencing primer. Questions and answers about using the hiseq 2500 system. Illumina hiseq 2000 sequencing system dna analyzer. Leverage this allinone library preparation and enrichment workflow, and design your preferred assay with a userfriendly online tool. Our existing truseq dna pcrfree library prep kits cat.
This means that when selecting illumina pipeline 1. Not for use in diagnostic procedures except as specifically noted. Hiseq sbs kit v4 kits contain readytoload reagents for accurately determining the dna sequence of each cluster on a flow cell using sequencing by synthesis sbs technology on enabled hiseq sequencing systems run in high output mode. Miseq fgx reagent kit components are rfidencoded and interact intelligently with the miseq fgx system to validate compatibility with forensic genomic applications. Value 2 b has special meaning and is used as a trim clipping. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could. Fc1201, fc1202, and fc1203 will transition to truseq dna pcrfree cat. Nextera rapid capture custom enrichment provides focused access to genomic regions of interest. Sureselectxt hs target enrichment system for illumina multiplexed sequencing 3 safety notices caution a caution notice denotes a hazard. A august 2011 this document and its contents are proprietary to illumina, inc. Thermo scientific museek library preparation kit, illumina compatible is designed for generation of high. Hiseq 2500 system resources access system literature.
In addition to onsite support, training courses are available to bring laboratory personnel quickly up to speed. The instructions in this document must be strictly and explicitly. This kit is an optional accessory for hiseq rapid cluster kits which require a cbot instrument, and is designed for use in the rapid run workflow of hiseq 2500 and 1500 sequencers. Miseq reagent kits v2 enable more output to simplify each miseq run. When you select to import illumina data and click next there is an option to use different quality score schemes at the bottom of the dialog see figure 6. These kits enable multiplexed sequencing with the introduction of 48 unique indexes, allowing mirna and small rna discovery and profiling throughput to match the unparalleled output of illumina sequencing. The hiseq 2500 system is the only highthroughput sequencing system that combines scalable output.
To provide greater flexibility in ordering single components for library preparation, we are moving to a modular configuration. System overview and instructions for operating and maintaining the hiseq 2500 system. Each of the 8 samples is represented by a different colour. The trusight myeloid sequencing panel covers 15 full genes exons only and 39 additional genes where oncogenic hotspots are covered, providing a comprehensive assessment of the key genes known to be involved in myeloid malignancies in a single test. View and download xante ilumina gl user manual online.
Hiseq rapid sbs kits v2 are designed for the rapid run mode of hiseq 2500 and 1500 sequencers. Miseq reagent kits v2 retain the same prefilled, readytouse reagent cartridges as the v1 kits, but offer improved chemistry to increase cluster density, decrease cycle time, and improve quality q scores. The hiseq 2500 system the hiseq 2500 system is the only highthroughput sequencing system that combines scalable output from 10 gb 1 tb with flexible run modes to support multiple applications and superior turnaround times. Truseq small rna library preparation kits illumina. The miseq fgx reagent kit uses prefilled, readytouse reagent cartridges, and offers the improved v3 chemistry to increase cluster density and read length as well as improve quality.
The fast protocol utilizes the mua transposase enzyme, which catalyzes simultaneous fragmentation of double. The hiseq rapid duo cbot sample loading kit allows maximum indexing freedom in rapid run mode by enabling individually addressable lanes. Featured hiseq 2500 products and services illumina. Illumina launches a new faster sequencer hiseq 2500. Riptide high throughput rapid dna library prep 96well plate format compatible with illumina sequencing for use with 50 ng of input dna contains reagents for 960 reactions b biotinterminated products bound to streptavidin beads c random primer d dual adapter library molecule e bead capture primer extension. Used illumina hiseq 2500 dna sequencer 8000876 dna.
Illumina scientists and engineers assist with ngs system installation and setup and train laboratory personnel. Sbs uses a reversibleterminator method, with fluorescently labeled nucleotides to detect single bases as they are incorporated into growing dna strands. This high capacity means that more samples can be sequenced simultaneously at a great depth, generating rich and meaningful data. Summary illumina next generation sequencing ngs platforms including miseq, genome analyzer iix, hiscansq, hiseq 2000, and hiseq 2500 1500 sequence dna molecules by massively parallel sequencing. Illumina uses onetrust, a privacy management software tool, to handle your request. Sureselectxt hs target enrichment system for illumina paired. Riptide high throughput rapid dna library prep 96well plate format compatible with illumina sequencing for use with 50 ng of input dna contains reagents for 960 reactions b biotinterminated products bound to streptavidin beads. Coolpad illumina full phone specifications, specs, manual user guide. You may receive emails through the onetrust system as your. The hiseq 30004000 sbs kits enable up to 1500 gb 1. Threestep procedure based on a novel, fragmentationfree and size selectionfree method. Miseq reagent kits v3 enable the highest output of all miseq kits. The v3 kits retain the same prefilled, readytouse reagent cartridges as the v1 and v2 kits, but offer improved chemistry to increase cluster density and read length as well as improve quality q scores.
The illumina hiseq 2500 is a powerful, flexible dna sequencer. High quality data using proven illumina sbs chemistry has made it the instrument of. Hiseq 2500 30004000, miseq, nextseq 500, miniseq and the hiseq x10x5 series. Illumina screening arrays have been widely adopted, with over 20 million samples ordered by a global community of users.
Sbs uses a reversibleterminator method, with fluorescently labeled nucleotides to detect single bases as. The illumina miseqdx cystic fibrosis 9variant assay is a qualitative in vitro diagnostic system used to simultaneously detect 9 clinically relevant cystic fibrosis diseasecausing mutations and variants of the cystic fibrosis transmembrane conductance regulator cftr gene in genomic dna isolated from human peripheral whole blood samples. At illumina, our goal is to apply innovative technologies to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. Also, the highoutput mode permits approximately five times read more. Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science.
The hiseq 2500 system harnesses proven illumina sbstechnology to deliver highly accurate data and robust performance for a broad range of applications. Up to 96 barcoded libraries suitable for illumina miseq, hiseq 1500, 2000 2500, 30004000 and novaseq sequencing instruments. Sureselectxt hs target enrichment system for illumina pairedend multiplexed sequencing library protocol agilent technologies 1. Instructions for using the truseq v2 cbot multiprimer rehybridization kit for primer rehybridization on a truseq v3 flow cell. Hiseq 2500 system hiseq 4000 system novaseq 6000 system analyze your data with.
The hiseq 2500 system the hiseq 2500 system is the only highthroughput sequencing system that combines scalability from 10600 gb of total output with flexible run. It is mission critical for us to deliver innovative, flexible, and scalable solutions to meet the needs of our customers. Chapter1overview introduction 1 additionalresources 1 instrumentcomponents 2 sequencingconsumablesoverview 5 introduction thehiseq. Visit the hiseq 2500, hiseq 1500, or hiseq 2000 support page on the illumina website. Coolpad illumina full phone specifications, specs, manual user guide my store. Illumina s reversible dye termination sequencing by synthesis technology is the foundation for their nextgen instruments. The hiseq 2500 system maintains the industryleading data quality of the hiseq 2000, but delivers daily throughput in excess of 100gb when used in rapid run mode. Learn how new v4 sbs kits allow for more reads and more data in less time on the hiseq 2500 sequencing system. It combines two different run modes, rapidrun and highoutput run, for one or two flow cells simultaneously. Leverage this allinone library preparation and enrichment workflow, and design your preferred assay with a user friendly online tool. Coolpad illumina full phone specifications manualuser. Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer genomics, and molecular diagnostics. Illumina tagment dna enzyme and buffer kits contain the tde1 tagment dna enzyme and td buffer tagment dna buffer. High concordance between hiseq 2000 and hiseq 2500 data.
The instructions in this document must be strictly and explicitly followed by. Sureselectxt hs target enrichment system for illumina. Museek library preparation kit, illumina compatible. Illumina services and support begins when the hiseq series is delivered. Provides an overview of instrument components and instructions for operating and maintaining the hiseq 2500. Hiseq 2500 specifications key performance parameters. Highquality data using proven illumina sbs chemistry has made it the instrument of choice for major genome centers and research institutions throughout the world. Introduction the hiseq 2500 has become the undisputed powerhouse of illumina. Quality scores in the illumina platform qiagen bioinformatics. Intel, ericsson, nokia and toshiba, and is supported by more than 2,500 organizations. The highoutput mode is ideal for larger studies with several samples or studies that require a greater depth of coverage. Recorded webinar april 2019 bcl2fastq is a linuxbased software that converts base call files generated from an illumina sequencing run to fastq files as well as demultiplex samples. Dual flow cellsthe hiseq 2500 is a dual flow cell system, which allows.
This document and its contents are proprietary to illumina, inc. The solid lines are the estimates for the libraries sequenced on the illumina hiseq 2500 platform, while the dotted lines are the estimates for the libraries sequenced on the bgiseq500. Hiseq 2500, 1500, and 2000 systems site prep guide 15006407. Highquality data using proven illumina sbs chemistry has made it the instrument of. Hiseq 1500 2500 sequencing systems the worlds most powerful sequencer, now faster and more flexible than ever. May 14, 2012 by nextgenseek the highthroughput sequencing leader illumina launched hiseq 2500 1500, as an update from its earlier version of highthroughput sequencer hiseq 2000, in early this year. Hiseq 2000 user guide iii notice this document and its contents are proprietary to ill umina, inc. Ericsson, nokia and toshiba, and is supported by more than 2,500 organizations. You may receive emails through the onetrust system as your request is processed.
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